![]() ![]() bZIP11 has been demonstrated to promote the expression of specific auxin-related genes by recruiting histone acetylation machinery to enhance chromatin accessibility 3, 11. The bZIP TFs bind DNA at cis-regulatory elements (CREs) known as G-boxes, which all have a conserved ACGT core flanked by cytosines or guanines (C/GACGTG/C) 10. ![]() It is estimated by DNA Affinity Purification with high throughput sequencing (DAP-seq) that bZIP11 contains DNA-binding sites in over 7,000 genes in Arabidopsis, which is nearly one third of the entire genes in the genome 9. In Arabidopsis thaliana (Arabidopsis), bZIP11 belongs to a group of five proteins involved in sugar responses, named the S 1 bZIP group 2, 4, 5, 6, 7, 8. Our study provides a resource for understanding how bZIP11 regulates the genome at the chromatin level and provides an example of the impact of a single transcription factor on a whole plant genome.īasic leucine zipper11 (bZIP11) is a transcription factor (TF) which regulates gene expression during low-energy conditions in plants and enables plants to adjust their metabolism, growth, and development to such unfavourable conditions 1, 2, 3. We identified, on average, 10,000 differentially accessible regions upon bZIP11 induction, corresponding to over 8,420 different genes out of the 25,000 genes present in the Arabidopsis genome. Here we addressed this by performing an ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) on Arabidopsis thaliana (Arabidopsis) leaf protoplasts to obtain a profile of chromatin patterning in response upon bZIP11 induction. Although previous results indicate that bZIP11 regulates chromatin accessibility based on evidence obtained from single genomic loci, to what extent this transcription factor regulates the chromatin landscape at the whole genome level remains unknown. We’ll be posting new videos regularly, so make sure you’re subscribed to our channel to see the latest V2 demos, tutorials, tips and tricks and more.Basic leucine zipper 11 (bZIP11) is a transcription factor that is activated under low energy conditions in plants and plays a crucial role in enabling plants to adapt to starvation situations. Head over to the Affinity YouTube channel and you’ll find a vast bank of video tutorials covering all the new features and core tools for each app. So don’t miss it-check out our purchasing options and buy Version 2 now at. The launch discount is available for a limited time, though. ![]() Whichever option you choose, it is a single one-off payment, no subscription. You can buy them individually for macOS, Windows or iPad or purchase an Affinity V2 Universal Licence covering the entire suite on all platforms-which is very cost-effective for those wishing to purchase more than one app or the same app on multiple platforms. To celebrate the release of Version 2, all Affinity apps-including Affinity Designer-are currently on sale with a substantial 40% launch discount! Get 40% off Affinity V2 for a limited time! Previously you could only apply them once. You can now apply multiple instances of Outline, Inner Shadow, Colour Overlay, Gradient Overlay and Outer Shadow to the same object. We’ve introduced a new straight-line mode and auto-close option to the Pencil Tool, the latter of which now allows you to automatically connect the final node with the first node by releasing the mouse button. Improved brush engine Pencil Tool improvements X-Ray viewĪ new view mode shows the makeup of your work, which is particularly useful for selecting a specific curve or object within a complex artwork. Select Same now has options for width, height and rotation, as well as to select hidden objects or not. New functionality allows you to scope your selection based on your current artboard or from within your current selection. Click-drag sets the scaling and rotation of the fill, with all changes happening instantly, in real time. When working with shapes and fill layers, you can now drop bitmap images from files, assets or other layers directly onto the fill icon in the Context Toolbar or your selected object. It provides a quick way to apply existing attributes to other areas within a design and can help you achieve a cohesive look throughout your work too. We’ve also added a new Style Picker Tool, which works in a similar way to the Colour Picker Tool, but allows you to copy and paste attributes such as Layer Opacity, Stroke, Fill, Layer Effects, Object Settings, Character Settings and Paragraph Settings. This functionality has many different applications, but it is particularly useful for placing elements that need to be arranged in a standardised layout. ![]() Quick Grid allows you to quickly and efficiently replicate shapes, picture frames and text frames in the shape of a grid. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |